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Cell and virus sensitivity studies with recombinant human alpha interferons.

M J Kramer, R Dennin, C Kramer

    Journal of Interferon Research
    |January 1, 1983
    PubMed
    Summary
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    Recombinant human alpha interferons (rIFN-alpha) show varied cell sensitivity across species. While some rIFN-alpha types protect mice against viral infections, others do not, indicating differential in vivo efficacy.

    Area of Science:

    • Virology
    • Immunology
    • Biochemistry

    Background:

    • Recombinant human alpha interferons (rIFN-alpha) are crucial antiviral agents.
    • Understanding cell sensitivity and in vivo efficacy is key to their therapeutic application.

    Purpose of the Study:

    • To investigate the cell sensitivity of various rIFN-alpha types across different cell lines.
    • To evaluate the in vitro antiviral activity and in vivo protective effects of rIFN-alpha.

    Main Methods:

    • Cell sensitivity assays using human, bovine, monkey, mouse, rabbit, and hamster cells.
    • In vitro antiviral activity testing against vesicular stomatitis virus, rhinoviruses, herpes simplex virus (HSV), Semliki Forest virus (SFV), and encephalomyocarditis virus (EMC).
    • In vivo protection studies in mice against viral infections.

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    Main Results:

    • Significant variations in rIFN-alpha sensitivity were observed across cell lines, with bovine cells showing high sensitivity to rIFN-alpha D.
    • All tested rIFN-alpha types demonstrated broad in vitro antiviral activity, with no significant differences in antiviral spectra.
    • In vivo, rIFN-alpha A showed limited protection, while rIFN-alpha D and A/D protected mice against EMC, SFV, and HSV infections.

    Conclusions:

    • Cell sensitivity to rIFN-alpha varies significantly, impacting their potential use in different species.
    • While in vitro antiviral activity is broad, in vivo efficacy differs, highlighting the importance of in vivo studies for therapeutic development.