A detailed physical and genetic map of two R.ColBM IncFIII plasmids

The cleavage and genetic maps of two closely related R.ColBM IncFIII plasmids, designated pSAS1201 and pSAS1203, are presented. Restriction analysis of both plasmids with SstI, EcoRI, Bg/II, XhoI, HindIII, and Sa/I indicated that the maps of these two plasmids are superimposable with the exception of a 1.70-MDa DNA segment absent in pSAS1203. Our results provide the first physical and genetic maps of plasmids belonging to the IncFIII incompatibility group.