Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Identification of cells in culture.

C S Stulberg, W D Peterson, W F Simpson

    American Journal of Hematology
    |January 1, 1976
    PubMed
    Summary
    This summary is machine-generated.

    Cell line cross-contamination is a significant issue in research. Over 14% of tested cell cultures were contaminated, and nearly 30% were misidentified, highlighting the need for routine monitoring.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Physiological observations upon larval Eustrongylides; influence of oxygen lack upon survival and glycogen consumption.

    Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.)·2010
    Same author

    Cell-blockade in canine distemper.

    Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.)·2010
    Same author

    Distamperoid virus interference in canine distemper.

    Science (New York, N.Y.)·2010
    Same author

    The effect of dosage on interference between distemper viruses.

    Journal of bacteriology·2010
    Same author

    Susceptibility of the gray fox to fox encephalitis.

    Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.)·2010
    Same author

    Physiological observations upon a larval Eustrongylides; the lethal mechanism of bacteria.

    The Journal of parasitology·2010
    Same journal

    High Proportion of PNH Type II Neutrophils Is Associated With Thrombosis in Patients Displaying a PNH Clone ≥ 1.

    American journal of hematology·2026
    Same journal

    Peripheral Blood Lymphocytosis Reflecting an Underlying Thymoma.

    American journal of hematology·2026
    Same journal

    Undetectable Hydroxyurea Levels in the Majority of Sickle Cell Disease Patients, Especially in Young Children.

    American journal of hematology·2026
    Same journal

    Myelodysplastic Syndromes: 2026 Update on Diagnosis, Risk-Stratification and Management.

    American journal of hematology·2026
    Same journal

    Signaling Mutations Negate the Favorable Impact of NPM1 Mutations in Older Patients With Newly Diagnosed Acute Myeloid Leukemia Treated With VEN/HMA.

    American journal of hematology·2026
    Same journal

    Comparative Efficacy of Intranasal, Intramuscular, and Intravenous Vitamin B12 Therapy for Hematological Recovery in Vitamin B12 Deficiency Anemia: A Randomized Controlled Trial.

    American journal of hematology·2026
    See all related articles

    Area of Science:

    • Cell Biology
    • Biotechnology
    • Research Integrity

    Background:

    • Laboratories often maintain multiple in vitro cell lines.
    • Cell line misidentification and cross-contamination can invalidate research findings.

    Purpose of the Study:

    • To assess the prevalence of cross-contamination and mislabeling in cell cultures.
    • To emphasize the importance of cell line authentication and monitoring.

    Main Methods:

    • Examined 246 cell cultures for cross-contamination and mislabeling.
    • Utilized species-specific antigens, isoenzyme electrophoresis, and chromosome analysis as identity markers.
    • Applied glucose-6-phosphate dehydrogenase and chromosome analyses for intraspecies identification of human cell lines.

    Related Experiment Videos

    Main Results:

    • 14% of submitted cultures were contaminated with cells of a different species.
    • 25% of human cell lines were identified as HeLa cell origin.
    • Overall, nearly 30% of cell lines were found to be incorrectly designated.

    Conclusions:

    • A significant proportion of cell lines are misidentified or cross-contaminated.
    • Routine monitoring of cell lines for species and intraspecies characteristics is crucial for research validity.
    • Implementing authentication protocols is essential to prevent data invalidation.