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Establishment and preliminary characterization of a cell line persistently infected with Newcastle disease virus
Abstract:
A murine osteosarcoma (OGS) cell line was permanently infected with Newcastle disease virus (NDV). The presence of NDV in the subsequent passages was demonstrated by haemadsorption and by immunofluorescence. Compared to the uninfected OGS line the persistently infected cells had a slightly reduced growth rate and they had a reduced sensitivity to several viruses, shown by several different methods. Interferon sensitivity was considerably reduced in the OGS-NDV cells, both the antiviral activity and the cell multiplication inhibitory activity.
Insights
Newcastle disease virus (NDV) persistently infected murine osteosarcoma cells, showing reduced growth and viral sensitivity. These OGS-NDV cells exhibited significantly decreased interferon sensitivity, impacting antiviral and cell growth inhibition.
Area of Science:
- Virology
- Oncology
- Immunology
Background:
- Osteosarcoma is a primary bone cancer.
- Newcastle disease virus (NDV) is an avian paramyxovirus with oncolytic potential.
- Persistent viral infections can alter host cell characteristics.
Purpose of the Study:
- To establish and characterize a murine osteosarcoma (OGS) cell line persistently infected with Newcastle disease virus (NDV).
- To investigate the biological and immunological consequences of persistent NDV infection in OGS cells.
Main Methods:
- Permanent infection of OGS cells with NDV.
- Confirmation of NDV presence using haemadsorption and immunofluorescence assays.
- Assessment of cell growth rates.
- Evaluation of OGS-NDV cell sensitivity to various viruses and interferon treatments.
Main Results:
- NDV was successfully established in OGS cells across multiple passages.
- Persistently infected OGS-NDV cells displayed a slightly reduced growth rate compared to uninfected OGS cells.
- OGS-NDV cells demonstrated reduced sensitivity to superinfection by several viruses.
- A significant reduction in interferon sensitivity was observed in OGS-NDV cells, affecting both antiviral and cell multiplication inhibitory activities.
Conclusions:
- Persistent NDV infection confers resistance to viral superinfection and modulates cellular responses to interferon in osteosarcoma cells.
- These findings suggest that NDV-infected osteosarcoma cells exhibit altered biological properties that may have implications for viral oncolysis and host-pathogen interactions.
