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Cell surface interactions in conjugation: Tetrahymena ciliary membrane vesicles.

B Love, M B Rotheim

    Molecular and Cellular Biology
    |April 1, 1984
    PubMed
    Summary

    Tetrahymena ciliary membrane vesicles influence cell pairing during conjugation. Vesicles of a different mating type enhance pairing, while same-type vesicles or addition at mixing inhibits it, suggesting dual interaction roles.

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    Area of Science:

    • Cell Biology
    • Reproductive Biology
    • Biochemistry

    Background:

    • Tetrahymena conjugation is a model for studying cell-cell recognition.
    • Mating type specificity is crucial for successful sexual reproduction in Tetrahymena.
    • Ciliary membrane vesicles play roles in cellular communication and interaction.

    Purpose of the Study:

    • To investigate the role of Tetrahymena ciliary membrane vesicles in regulating cell pairing during conjugation.
    • To determine if vesicle interaction with cells is mating type-specific.
    • To elucidate the mechanisms underlying vesicle-mediated enhancement or inhibition of cell pairing.

    Main Methods:

    • Treatment of Tetrahymena cells with ciliary membrane vesicles of different mating types prior to or during mixing for conjugation.

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  • Observation and quantification of cell pairing efficiency and prepairing periods.
  • Varying vesicle concentration and timing of addition to assess dose-dependent and time-dependent effects.

    • Main Results:

    • Pretreatment with heterotypic vesicles (different mating type) enhanced cell pairing and reduced the prepairing period.
    • Pretreatment with homotypic vesicles (same mating type) did not enhance pairing.
    • Addition of vesicles at the time of mixing inhibited cell pairing in a concentration-dependent manner.

    Conclusions:

    • Tetrahymena ciliary membrane vesicles mediate cell pairing through at least two distinct mechanisms.
    • Vesicles can act as substitutes for cell-cell interactions in initiating pairing.
    • Vesicles can also compete with cell adhesion sites, inhibiting pairing under certain conditions, potentially analogous to hormone-receptor signaling.