Related Experiment Video
Updated: Aug 11, 2026

Characterization of Membrane Transporters by Heterologous Expression in E. coli and Production of Membrane Vesicles
Published on: December 31, 2019
Transport of aminoglycosides in Escherichia coli
Abstract:
Uptake of nine aminoglycosides was studied in E. coli K12 and mutants being defective in the outer membrane proteins OmpF and OmpC or in ATPase (uncA). OmpF/OmpC as well as uncA mutants did not take up the aminoglycosides; transport in wild type cells was cAMP dependent. The specific binding of phages using the OmpF and OmpC proteins as receptors was strongly reduced by the aminoglycosides or by other polycationic peptides. Thus it may be assumed that the initial step of aminoglycoside transport is their electrostatic binding to the anionic porins, especially to the ompF porin, followed by a facilitated permeation through the outer membrane. As the synthesis of this porin is under cAMP control, aminoglycoside transport is cAMP dependent as well. The fact that the uncA mutant did not take up the aminoglycosides to any appreciable extent indicates that the cytoplasmic membrane is involved in aminoglycoside transport too.
More Related Videos
05:06Characterizing Multidrug Efflux Systems in Acinetobacter baumannii Using an Efflux-Deficient Bacterial Strain and a Single-Copy Gene Expression System
Published on: January 5, 2024
09:22Amikacin Protection Assay for Quantification and Visualization of Escherichia coli Cell Invasion
Published on: July 3, 2026
Related Concept Videos
ABC Transporters: Exporter
ABC Transporters: Importer
In bacteria, based on the number of transmembrane helices and the chemical nature of their substrates, the ABC importers can be divided into three types:
Drug Elimination by Renal Route: Tubular Secretion
Carrier-Mediated Transport
Active transport involves two types of membrane-spanning transporters: uptake and efflux. Uptake transporters are expressed in the small...
Stringent Response in E. coli
Inhibitors of Bacterial Protein Synthesis