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Comparison of six methods for detecting human rotavirus in stools
European Journal of Clinical Microbiology
|April 1, 1984
Summary
Comparing rotavirus detection methods, Enzygnost showed the highest sensitivity (98%) in human faecal samples. Rotalex is a valuable, sensitive screening tool for rotavirus in children with diarrhea.
Area of Science:
- Virology
- Clinical Diagnostics
- Microbiology
Background:
- Rotavirus is a leading cause of severe diarrheal disease in infants and young children worldwide.
- Accurate and sensitive detection of rotavirus in faecal samples is crucial for diagnosis and epidemiological studies.
Purpose of the Study:
- To compare the sensitivity and utility of six different diagnostic methods for detecting rotavirus in human faecal samples.
- To identify the most effective method for rotavirus detection in pediatric patients.
Main Methods:
- Six rotavirus detection assays were evaluated: electron microscopy, immune electron microscopy, immunofluorescence in cell culture, Rotazyme (enzyme immunoassay), Enzygnost (enzyme immunoassay), and Rotalex (latex agglutination test).
- Faecal specimens were collected from 112 children presenting with diarrhea.
- Relative sensitivities of each assay were calculated.
Main Results:
- The sensitivities ranged from 84% (electron microscopy) to 98% (Enzygnost).
- Enzygnost demonstrated the highest sensitivity (98%), followed by immune electron microscopy (93%).
- Rotalex (88% sensitivity) was found to be valuable for screening smaller numbers of samples, with no false positives observed for enzyme immunoassays or Rotalex.
Conclusions:
- Enzygnost is the most sensitive assay for detecting human rotavirus in faecal samples.
- Rotalex offers a practical and reliable option for initial screening of rotavirus in clinical settings.
- Enzyme immunoassays and Rotalex provide reliable results without false positives.