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Updated: Jul 23, 2026

Detection of the Genome and Transcripts of a Persistent DNA Virus in Neuronal Tissues by Fluorescent In situ Hybridization Combined with Immunostaining
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Detection of the Genome and Transcripts of a Persistent DNA Virus in Neuronal Tissues by Fluorescent In situ Hybridization Combined with Immunostaining

Published on: January 23, 2014

Unusual membrane bound intranuclear inclusions in human fibroblasts infected with herpes simplex

C N Sun, P N Morgan

    Experimental Pathology
    |January 1, 1984
    PubMed

    Insights

    Herpes simplex virus (HSV) was identified in autopsy brain tissue. The study observed HSV replication and unique nuclear inclusions within infected human cells.

    Area of Science:

    • Neuropathology
    • Virology
    • Cell Biology

    Background:

    • Herpes simplex virus (HSV) is a significant human pathogen.
    • Understanding HSV's interaction with host cells is crucial for developing antiviral strategies.

    Observation:

    • Autopsy brain tissue from a 55-year-old male tested positive for HSV antigen.
    • Inoculation of tissue into human diploid fibroblasts revealed HSV particles within cell nuclei.
    • Viral replication involved nuclear membranes, endoplasmic reticulum, and various cellular membranes.

    Findings:

    • HSV particles were observed in multiple developmental stages within the nucleus.
    • Nuclear membrane reduplication was a prominent feature of infected cells.
    • Unique, membrane-bound, dense, rounded inclusions were found within the nucleus, potentially forming within the nuclear membrane.

    Implications:

    • This study provides detailed ultrastructural insights into HSV replication in human brain tissue.
    • The findings highlight novel aspects of HSV-induced cellular pathology.
    • Further research into these inclusions may reveal new therapeutic targets for HSV infections.

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