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Related Experiment Videos

From bone lining cell to osteocyte--an SEM study.

D N Menton, D J Simmons, S L Chang

    The Anatomical Record
    |May 1, 1984
    PubMed
    Summary
    This summary is machine-generated.

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    Bone lining cells (BLCs) transform into osteocytes by embedding within the bone matrix, forming cell processes and lacunae. This study details their dynamic morphological changes during osteocyte development and lacuna formation.

    Area of Science:

    • Cell Biology
    • Bone Biology
    • Histology

    Background:

    • Endosteal bone lining cells (BLCs) are crucial for bone remodeling.
    • Their transformation into osteocytes is a key process in bone formation.

    Purpose of the Study:

    • To describe the SEM appearance of rat endosteal bone lining cells (BLCs).
    • To detail the morphological changes of BLCs as they become osteocytes.
    • To elucidate the process of lacuna construction by developing osteocytes.

    Main Methods:

    • Scanning Electron Microscopy (SEM) was used to visualize BLCs.
    • Gentle osteoid digestion with 0.25% collagenase to reveal cell morphology.
    • Analysis of cell-matrix interactions and lacuna formation.

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    Main Results:

    • BLCs develop complex branched processes upon leaving the BLC compartment.
    • These processes connect with those of adjacent osteocytes.
    • Mature osteoblasts form lacunae by depositing an asymmetric perimeter of woven collagen fibrils.
    • Perilacunar matrix collagen shows tangential/circular orientation, while distal matrix shows parallel orientation.
    • Lacunae mineralize rapidly upon completion, making only forming lacunae recognizable.

    Conclusions:

    • The study provides a detailed morphological description of BLCs transitioning into osteocytes.
    • It clarifies the process of osteocyte lacuna formation and its relationship with matrix deposition.
    • These findings enhance our understanding of bone cell differentiation and matrix organization.