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In vitro transformation by bovine papillomavirus.
The Journal of Investigative Dermatology
|July 1, 1984
Summary
Bovine papillomavirus (BPV) induces cell proliferation and transformation in mouse cells. Specific DNA segments within BPV are crucial for this transforming activity, with one segment encoding the function and another acting as a control element.
Area of Science:
- Molecular Biology
- Virology
- Oncology
Background:
- Bovine papillomavirus (BPV) serves as a model for studying papillomavirus-induced cell proliferation and tumorigenesis.
- BPV infection can lead to the transformation of mouse tissue culture cells.
Purpose of the Study:
- To investigate the specific DNA sequences of BPV responsible for inducing tumorigenic transformation in mouse cells.
- To delineate the minimal DNA fragments required for BPV-mediated cell transformation.
Main Methods:
- Studied tumorigenic transformation of mouse cells using bovine papillomavirus (BPV).
- Analyzed the transforming capacity of BPV DNA fragments and deletion mutants.
- Utilized a retroviral control element to activate specific BPV DNA segments.
Main Results:
- BPV-induced transformed cells maintain the viral DNA as extrachromosomal copies.
- Transformation capacity was localized to a 69% subgenomic fragment of BPV DNA.
- Two discontinuous DNA segments are essential for transformation: a 5' control element and a 3' transforming sequence.
Conclusions:
- The 3' segment of the BPV DNA fragment encodes the transforming function.
- A retroviral control element can activate the 2.3-kb transforming segment of BPV DNA.