Related Experiment Videos
[Chromium binding to leukocytes]
The experiment has been done on 63 patients affected by allergic contact dermatitis and sensitized to potassium dichromate and on 76 non allergic subjects to control: if leukocytes (separated in emagel), mononuclear cells (separated in lymphoprep) and T-lymphocytes (separated through rosettes with neuraminidase) bind the 51Cr, if this binding depends on the valence (Cr3+ and Cr6+) and on the type of cells and if it is conditioned by the hypersensitivity. Through incubation of the cells with the tracing at 4 degrees C and in different times, it has been demonstrated that the binding takes place, it depends on the valence and, in certain conditions it is bigger for the mononuclear cells and the T-lymphocytes in allergic subjects.
The experiment has been done on 63 patients affected by allergic contact dermatitis and sensitized to potassium dichromate and on 76 non allergic subjects to control: if leukocytes (separated in emagel), mononuclear cells (separated in lymphoprep) and T-lymphocytes (separated through rosettes with neuraminidase) bind the 51Cr, if this binding depends on the valence (Cr3+ and Cr6+) and on the type of cells and if it is conditioned by the hypersensitivity. Through incubation of the cells with the tracing at 4 degrees C and in different times, it has been demonstrated that the binding takes place, it depends on the valence and, in certain conditions it is bigger for the mononuclear cells and the T-lymphocytes in allergic subjects.