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Rapid method for detection, identification, and susceptibility testing of enteric pathogens.

C E Stager, E Erikson, J R Davis

    Journal of Clinical Microbiology
    |January 1, 1983
    PubMed
    Summary

    A rapid biochemical screening method accurately identified enteric pathogens in 4-6 hours, with the AutoMicrobic system Enterobacteriaceae-Plus Biochemical Card (AMS-EBC+) achieving 96.8% accuracy for presumptive pathogens.

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    Area of Science:

    • Clinical Microbiology
    • Diagnostic Bacteriology
    • Infectious Diseases

    Background:

    • Accurate and rapid identification of enteric pathogens is crucial for timely clinical intervention and infection control.
    • Traditional biochemical methods for enteric pathogen identification can be time-consuming, delaying diagnosis and treatment.

    Purpose of the Study:

    • To evaluate a rapid biochemical screening method for the identification of enteric pathogens.
    • To assess the performance of the AutoMicrobic system Enterobacteriaceae-Plus Biochemical Card (AMS-EBC+) in identifying presumptive enteric pathogens.

    Main Methods:

    • A panel of 307 suspected enteric pathogen isolates was subjected to rapid biochemical screening (4-6 hours) and traditional 24-hour reference methods.
    • Presumptive pathogens identified by rapid screening were further analyzed using the AutoMicrobic system Enterobacteriaceae-Plus Biochemical Card (AMS-EBC+).

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  • Antimicrobial susceptibility testing was performed using agar diffusion and compared with AutoMicrobic system general susceptibility card results.

    • Main Results:

    • The rapid screening method identified 87 (28.3%) isolates as presumptive enteric pathogens within 4-6 hours.
    • The AMS-EBC+ correctly identified 61 out of 63 confirmed enteric pathogens (96.8%) from the presumptive group.
    • Minor discrepancies in antimicrobial susceptibility testing were observed for some Salmonella and Shigella isolates with tetracycline, but no major or very major discrepancies were noted.

    Conclusions:

    • The rapid biochemical screening method, coupled with the AMS-EBC+, offers a highly accurate and efficient approach for the presumptive identification of enteric pathogens.
    • This rapid method can significantly reduce the time to identification compared to traditional 24-hour biochemical assays.
    • The study validates the utility of the AMS-EBC+ for routine clinical microbiology laboratories handling enteric pathogen detection.