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Allophenic mice produced from embryos aggregated with antibody.

J Palmer, M J Dewey

    Experientia
    |February 15, 1983
    PubMed
    Summary

    Producing allophenic mice is challenging as embryos often separate. Pre-treating embryos with rabbit anti-mouse serum before aggregation prevents separation, enabling successful allophenic mouse production.

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    Area of Science:

    • Developmental biology
    • Embryology
    • Genetics

    Background:

    • Allophenic mice are valuable models for studying gene function and developmental processes.
    • A key challenge in producing allophenic mice is preventing the dissociation of aggregated preimplantation embryos.

    Purpose of the Study:

    • To describe a novel method for improving the efficiency of allophenic mouse production.
    • To overcome the issue of blastomere dissociation during embryo aggregation.

    Main Methods:

    • Pre-treatment of one embryo with rabbit anti-mouse serum before aggregation.
    • Aggregation of pre-treated and untreated preimplantation embryos.
    • Assessment of blastocyst formation and viability.

    Main Results:

    • The antibody treatment effectively prevents the rolling apart of aggregated embryos.
    • Blastocyst formation proceeds normally in antibody-treated embryos.
    • Numerous allophenic mice were successfully produced using this new procedure.

    Conclusions:

    • Rabbit anti-mouse serum pre-treatment is an effective solution to enhance allophenic mouse production.
    • This method simplifies the process and increases the success rate of generating chimeric embryos.

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