Related Experiment Video

Updated: Aug 8, 2026

On-chip Isotachophoresis for Separation of Ions and Purification of Nucleic Acids
10:32

On-chip Isotachophoresis for Separation of Ions and Purification of Nucleic Acids

Published on: March 2, 2012

Isotachophoretic electrodesorption of proteins from an affinity adsorbent on a microscale

V Kasicka, Z Prusík

    Journal of Chromatography
    |March 11, 1983
    PubMed

    Cationic isotachophoresis was used for the desorption of mouse monoclonal antibody to transferrin strongly affinity-bonded to transferrin immobilized on polyethyleneglycol terephthalate powder. The electrodesorption under nondestructive conditions was effected in the capillary isotachophoresis apparatus of our own construction which was equipped with an adsorption element. The electrodesorption is on-line connected with quantitative isotachophoretic analysis of the antibody desorbed. Only a few tens of microliters of the affinity adsorbent and several nanomoles of the antibody are needed for the characterization of the capacity of the affinity adsorbent and the conditions of adsorption and desorption.

    More Related Videos

    Protein Purification-free Method of Binding Affinity Determination by Microscale Thermophoresis10:22

    Protein Purification-free Method of Binding Affinity Determination by Microscale Thermophoresis

    Published on: August 15, 2013

    Use of Microscale Thermophoresis to Measure Protein-Lipid Interactions04:45

    Use of Microscale Thermophoresis to Measure Protein-Lipid Interactions

    Published on: February 10, 2022

    Related Concept Videos

    Two-dimensional Gel Electrophoresis01:22

    Two-dimensional Gel Electrophoresis

    Two-dimensional gel electrophoresis is a high-resolution protein separation method first introduced by O' Farrell and Klose in 1975. This method involves protein separation by two dimensions, mass and charge, making it more accurate than one-dimensional gel electrophoresis.
    The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
    Biological samples, such as  cells...
    Potentiometry: Membrane Electrodes01:15

    Potentiometry: Membrane Electrodes

    Membrane electrodes, also known as p-ion electrodes, use membranes that selectively interact with free analyte ions, generating a potential difference across the membrane. The resulting membrane potential, known as the asymmetry potential, is not zero even when analyte concentrations on both sides of the membrane are equal. The membrane's response is typically not selective to a single analyte but proportional to the concentration of all ions in the sample solution capable of interacting at the...
    Analyte Adsorption and Distribution01:09

    Analyte Adsorption and Distribution

    In certain chromatographic separations, solutes transfer between the mobile phase and the stationary phase via sorption, which typically refers to the process of adsorption. For many chromatographic systems, the sorption process often depends on the polarity of the compounds—an expression of the overall dipole moment within the molecule. During the separation process, there is competition between the solute and solvent for adsorption to the stationary phase. Highly polar compounds and solvents...
    Ion-Exchange Chromatography01:09

    Ion-Exchange Chromatography

    Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...
    Affinity Chromatography01:03

    Affinity Chromatography

    Affinity chromatography is a powerful technique extensively utilized for separating and purifying specific biomolecules from complex mixtures. It capitalizes on the highly selective binding between an analyte and its counterpart, such as antibody-antigen interactions. The counterpart is immobilized on the stationary phase, forming an affinity column. The stationary phase typically consists of solid support, such as agarose or porous glass beads, immobilizing the affinity ligand. The mobile...
    Capillary Electrophoresis: Applications01:30

    Capillary Electrophoresis: Applications

    Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
    Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...