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A method for the isolation of intact, viable forespores from Bacillus species using high pressure
Analytical Biochemistry
|February 15, 1983
Abstract:
Large-scale preparation of Bacillus forespores was performed using nitrogen gas under high pressure to force an osmotically stable spheroplast suspension through a micrometer needle valve. Ninety-nine percent recovery of intact viable forespores at various stages of sporulation was achieved with a variety of Bacillus species. The isolated forespores are stable for up to 7 days when kept under nongerminating conditions.
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