Escherichia coli minichromosomes containing the pSC101 partitioning locus are stably inherited

The par region of pSC101, required in cis to promote its stable inheritance, was joined, in combination with the tetr determinant of pBR325, to large and small minichromosomes. These hybrid minichromosomes were examined for stability and found to be no more stable than their parent minichromosomes. Indeed, one recombinant plasmid, pEH21, showed reduced stability, which was not attributable to a reduced copy number. Neither pEH21 nor pEH22, a plasmid composed of the same DNA arranged differently, was stabilized by the presence of a Par+ pSC101 derived replicon in the same cell. We conclude that the par region of pSC101 does not stabilize minichromosomes.