Neutrophil iodination micro-method as an index of neutrophil and opsonic function

A neutrophil iodination micro-method has been developed for assessment of neutrophil and opsonic function in any serum/micro-organism combination. The test is performed in micro-titre trays and thus requires relatively small amounts of reagents and numbers of cells. The variables of the test have been explored and the effect of immunization with different organisms on opsonin levels defined. The versatility of the test has been demonstrated in its ability to measure both heat labile and heat stable opsonins in normal as well as immune serum, for a number of different micro-organisms. The method is simple, rapid and a large number of tests can be performed at the one time.