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Simple technique to facilitate tissue sampling for electron microscopy.

J H Martin, P B Marcus

    Ultrastructural Pathology
    |March 1, 1983
    PubMed
    Summary
    This summary is machine-generated.

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    This study presents a simple method for selecting tissue samples for electron microscopy after initial formalin fixation. It enables efficient and cost-effective isolation of representative specimens for detailed ultrastructural analysis.

    Area of Science:

    • Histology
    • Electron Microscopy
    • Pathology

    Background:

    • Paraffin sections often reveal the need for electron microscopy after initial formalin fixation.
    • Large specimens pose challenges for isolating representative samples for electron microscopy.
    • Current methods can be time-consuming and expensive.

    Purpose of the Study:

    • To describe a simple, quick, and inexpensive method for overcoming the sampling problem in electron microscopy.
    • To facilitate the selection of appropriate tissue for ultrastructural analysis from formalin-fixed specimens.

    Main Methods:

    • Specimens are taken directly from formalin fixation.
    • Thin slices are prepared by hand using a blade.
    • Slices are stained with methylene blue and examined wet under a light microscope.

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  • Representative tissue is identified and isolated by micro-dissection for further electron microscopy processing.

    • Main Results:

    • The described technique allows for rapid identification and isolation of suitable tissue samples.
    • It overcomes the difficulty of sampling large, formalin-fixed specimens.
    • The method is cost-effective and requires minimal specialized equipment.

    Conclusions:

    • This technique provides an efficient solution for electron microscopy sample selection from formalin-fixed tissues.
    • It is particularly useful in laboratories routinely using formalin for both light and electron microscopy.
    • The method enhances the feasibility of ultrastructural studies by simplifying the initial sampling process.