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Human monocomponent insulin. Chemistry and characteristics.
Acta Medica Scandinavica. Supplementum
|January 1, 1983
Summary
Novo Research Institute developed a method to produce human insulin from porcine insulin using enzymatic conversion. This process extends existing manufacturing techniques for purified insulins.
Area of Science:
- Biochemistry
- Pharmaceutical Science
Background:
- Review of primary structures of various insulins.
- Summary of human insulin properties, including identification, purity, potency, and immunogenicity.
Purpose of the Study:
- To describe the enzymatic conversion method for manufacturing human insulin.
- To highlight the extension of current Novo purified insulin production processes.
Main Methods:
- Enzymatic conversion reaction to substitute B30 alanine with threonine in porcine insulin.
- Leveraging existing manufacturing processes for Novo purified insulins.
Main Results:
- Successful substitution of B30 alanine with threonine in porcine insulin.
- Production of human insulin through an extended enzymatic process.
Conclusions:
- The enzymatic conversion method provides a viable route for human insulin manufacturing.
- This process builds upon established techniques for producing purified insulins.