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Related Experiment Videos

Test for temporal or spatial restrictions in gene product function during the cell division cycle.

S K Dutcher, L H Hartwell

    Molecular and Cellular Biology
    |July 1, 1983
    PubMed
    Summary

    Investigating cell division cycle (CDC) genes in yeast revealed that some gene products function with spatial or temporal restrictions. This study used heterokaryons to show complementation differences, identifying specific CDC genes with restricted functions.

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    Area of Science:

    • Molecular Biology
    • Genetics
    • Cell Biology

    Background:

    • Gene complementation can depend on the intracellular relationship between functional and nonfunctional genes.
    • Cell division cycle (CDC) gene products in Saccharomyces cerevisiae regulate specific cell division stages, suggesting potential temporal or spatial functional restrictions.

    Purpose of the Study:

    • To investigate whether CDC gene products exhibit temporal or spatial functional restrictions.
    • To compare gene complementation within the same nucleus (diploids) versus different nuclei (heterokaryons).

    Main Methods:

    • Utilized Saccharomyces cerevisiae heterokaryons to assess complementation of temperature-sensitive cdc mutants.
    • Monitored complementation by analyzing the production of cdc haploid progeny (cytoductants) at restrictive temperatures.

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  • Compared complementation efficiency between CDC X cdc matings.

    • Main Results:

    • Most tested cdc mutants (33) showed complementation in heterokaryons.
    • cdc28 and cdc37 gene products appear to have spatial or temporal restrictions, as indicated by inefficient cytoductant production.
    • The cell division defect of cdc4 mutants was complemented in heterokaryons, but the karyogamy defect was not.

    Conclusions:

    • The study provides evidence for spatial or temporal restrictions in the function of certain CDC gene products.
    • Complementation assays in heterokaryons are effective for revealing gene product localization and function.
    • CDC4 gene function is differentially restricted, affecting cell division but not karyogamy in heterokaryotic complementation.