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Comparative study of different urokinase preparations.

W T Millar, J F Smith

    Thrombosis Research
    |June 1, 1983
    PubMed
    Summary

    The urokinase preparation with the lowest amidolytic activity showed the highest plasminogen activator activity. This suggests caution when comparing urokinase using amidolytic assays for clinical effectiveness.

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    Area of Science:

    • Biochemistry
    • Pharmacology
    • Thrombolytic Therapy

    Background:

    • Urokinase is a thrombolytic agent used to treat blood clots.
    • Assessing urokinase activity is crucial for determining its clinical efficacy.
    • Different urokinase preparations may exhibit varying enzymatic properties.

    Purpose of the Study:

    • To compare the amidolytic and plasminogen activator activities of three different urokinase preparations.
    • To investigate the relationship between amidolytic activity and plasminogen activator activity in urokinase.
    • To evaluate the suitability of amidolytic assays for predicting clinical effectiveness of urokinase.

    Main Methods:

    • Assay of amidolytic activity for each urokinase preparation.
    • Assay of plasminogen activator activity for each urokinase preparation.
    • Comparative analysis of the results from both activity assays.

    Main Results:

    • One urokinase preparation demonstrated significantly higher plasminogen activator activity compared to others.
    • This highly active preparation exhibited the least amidolytic activity.
    • A potential inverse correlation between amidolytic and plasminogen activator activity was observed.

    Conclusions:

    • The urokinase preparation with the lowest amidolytic activity may possess superior clinical effectiveness.
    • Standard amidolytic assays may not accurately reflect the true plasminogen activator potential of urokinase.
    • Caution is advised when using amidolytic assays for comparative assessment of urokinase preparations for clinical use.

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