Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[Acidification kinetics in macrophage phagosomes based on flow cytometric data].

A V Filatov, A V Khramtsov, E P Senchenkov

    Tsitologiia
    |June 1, 1983
    PubMed
    Summary

    This study reveals two distinct acidification phases during macrophage phagocytosis of Candida albicans yeast cells. These pH changes are crucial for the cellular uptake and processing of the yeast.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    [Dysplastic nevi: difficulties in diagnosis].

    Arkhiv patologii·2025
    Same author

    [Spectrum of morphological changes in chronic spontaneous urticaria and urticarial vasculitis].

    Arkhiv patologii·2025
    Same author

    High Heterogeneity of Virus-Neutralizing and RBD-Binding Activities of COVID-19 Convalescent Sera.

    Molecular biology·2022
    Same author

    [High Heterogeneity of Virus-Neutralizing and RBD-Binding Activities of COVID-19 Convalescent Sera].

    Molekuliarnaia biologiia·2022
    Same author

    CD44-Associated Tn Antigen as a New Biomarker of Tumor Cells with Aberrant Glycosylation.

    Biochemistry. Biokhimiia·2020
    Same author

    Next-Generation Techniques for Discovering Human Monoclonal Antibodies.

    Molecular biology·2020

    Area of Science:

    • Immunology
    • Cell Biology
    • Microbiology

    Background:

    • Macrophages play a critical role in the innate immune system, engulfing and eliminating pathogens like Candida albicans.
    • Phagocytosis is a key cellular process involving the internalization of large particles, leading to the formation of phagosomes and subsequent fusion with lysosomes.
    • Understanding the dynamic changes within phagocytic cells during pathogen interaction is crucial for developing effective immune responses.

    Purpose of the Study:

    • To investigate the temporal changes in intracellular pH during macrophage phagocytosis of Candida albicans.
    • To characterize the distinct phases of acidification associated with yeast cell interaction and internalization.
    • To elucidate the mechanisms underlying phagosomal acidification using pharmacological inhibitors.

    Related Experiment Videos

    Main Methods:

    • Flow cytofluorimetry was employed to measure intracellular pH changes in macrophages.
    • Candida albicans yeast cells were labeled with fluorescein isothiocyanate (FITC) for tracking.
    • Macrophage-yeast cell interactions were analyzed, including surface absorption and phagolysosome formation.
    • The effects of ammonium chloride (NH4Cl) and cytochalasin B on acidification were assessed.

    Main Results:

    • Two distinct phases of acidification were observed during phagocytosis.
    • The first phase, with a pH of 6.3 +/- 0.5, correlates with yeast cell surface absorption.
    • The second phase, with a pH of 4.5 +/- 0.5, corresponds to yeast cell incorporation into phagolysosomes.
    • Ammonium chloride treatment abolished acidification, while cytochalasin B inhibited the entire acidification process.

    Conclusions:

    • Macrophage phagocytosis of Candida albicans involves a biphasic intracellular acidification process.
    • The initial acidification is linked to cell surface adherence, and the subsequent, more pronounced acidification occurs within phagolysosomes.
    • Phagosomal acidification is an active process dependent on cellular mechanisms, as evidenced by the inhibitory effects of cytochalasin B.