Immunocytochemical localization of acrosin in boar spermatozoa

Acrosin was localized on the acrosomal membranes of boar sperm by immunofluorescence and immunoelectron microscopy. Indirect labeling of sperm head sections was performed with an Imposil (iron-dextran) antibody conjugate. The results of electron microscopy indicated a preferential deposition of the antiacrosin antibodies on the outer acrosomal membrane, although a lesser deposition was also found on the inner acrosomal membrane. No labeling was detected in the acrosomal content when such material was still present.