Insulin increases entrance of 2-deoxy-D-[3H]glucose in isolated rat brain microvessels

Insulin in a dose of 0.5 U ml-1 in the incubation medium did not change ATP and phosphocreatine contents in the isolated rat brain microvessels, when energy production was supported by 5.5 mmol l-1 glucose. However, the entrance of non-metabolizable glucose analogue, 2-deoxy-D-[3H]glucose into the microvessel cells was strongly enhanced, almost 18-fold.