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Somatic cell genetics and flow cytometry.

M E Kamarck, J A Barbosa, L Kühn

    Cytometry
    |September 1, 1983
    PubMed
    Summary
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    Researchers used cell sorting to analyze human cell surface genes in mouse cells. This technique allows for the manipulation and monitoring of human cell surface molecules using flow cytometry.

    Area of Science:

    • Genetics
    • Immunology
    • Cell Biology

    Background:

    • Cell surface molecules play crucial roles in cellular functions and interactions.
    • Somatic cell genetics offers powerful tools for studying gene function and cellular processes.
    • Understanding the human cell surface is vital for numerous biological and medical applications.

    Purpose of the Study:

    • To review and demonstrate the application of cell sorting for somatic cell genetic analysis of the human cell surface.
    • To highlight the utility of flow cytometry and sorting for monitoring and manipulating human genes in a host system.
    • To establish a framework for dissecting the genetic basis of human cell surface characteristics.

    Main Methods:

    • Introduction of human genes encoding cell surface molecules into mouse host cells.

    Related Experiment Videos

  • Utilizing indirect immunofluorescence for the detection of human gene products on viable cells.
  • Employing flow cytometry and cell sorting for gene monitoring and manipulation.

    • Main Results:

    • Demonstrated successful introduction and expression of human cell surface genes in mouse cells.
    • Established the capability to detect and quantify human gene products using immunofluorescence and flow cytometry.
    • Showcased the effectiveness of cell sorting for isolating and analyzing cells based on human cell surface marker expression.

    Conclusions:

    • Cell sorting is an effective method for developing somatic cell genetic analyses of the human cell surface.
    • Flow cytometry and sorting provide robust tools for the manipulation and study of human cell surface genes.
    • This approach facilitates a deeper understanding of human cell surface molecule genetics and function.