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[Prolylendopeptidase activity in various animal organs]
Biulleten' Eksperimental'Noi Biologii I Meditsiny
|November 1, 1983
Summary
Prolylendopeptidase activity varies across cattle tissues, with the highest concentration found in the brain and the lowest in the spleen. Molecular weights and inhibitor effects were analyzed in key organs.
Area of Science:
- Biochemistry
- Enzymology
- Comparative Physiology
Background:
- Prolylendopeptidase is an enzyme involved in protein degradation.
- Understanding its tissue distribution and characteristics is crucial for various biological processes.
Purpose of the Study:
- To investigate the distribution of prolylendopeptidase activity in different cattle tissues.
- To determine the molecular weights and inhibitor profiles of prolylendopeptidase in selected tissues.
Main Methods:
- Enzyme activity assay using Z-Gly-Pro-pNA hydrolysis.
- Gel filtration chromatography (Ultrogel AcA 44) for molecular weight determination.
- Inhibitor studies using p-chloromercuribenzoate (PCMB).
Main Results:
- Highest specific prolylendopeptidase activity was detected in the cerebral cortex, with the lowest in the spleen.
- Molecular weights of proteins with this activity ranged from 57,000 to 66,000 daltons in adenohypophysis, liver, kidneys, skeletal muscle, and spleen.
- Complete inhibition by PCMB was observed in adenohypophyseal extracts, while spleen, skeletal muscle, and liver showed 85% inhibition, and kidneys showed 65% inhibition.
Conclusions:
- Prolylendopeptidase activity exhibits significant tissue-specific distribution in cattle.
- The enzyme's molecular weight and sensitivity to PCMB vary across different tissues, suggesting potential differences in its structure or environment.