Indirect immunoperoxidase test for the diagnosis of paratuberculosis

An indirect immunoperoxidase procedure for the diagnosis of paratuberculosis was described. Formalin-fixed ileocecal tissue containing large numbers of Mycobacterium paratuberculosis organisms was used as the source of antigen. Goat anti-bovine immunoglobulin G labeled with peroxidase was used as the conjugate in the test system. The method is relatively simple to do and may prove to be valuable as a routine screening test.