[Determination of the mitotic index in tumors by using cell suspensions]

A new variant of the method for mitotic index determination is offered. For this, a tumor cell suspension is obtained by treating the tumor with a 0.3% trypsin solution. Then the cells are transferred to a hypotonic solution where these undergo lysis and, in result, the nuclei are seen to be released in the medium. The ratio of nuclei being in meta- and anaphase is determined. Determination of the mitotic index in tumors using this method allows to avoid a technical error associated with the fact that the proliferative activity is not uniform in different parts of the tumor.