Comparative studies of Trypanosoma cruzi and T. cruzi-like stocks from different South American countries using lectins

The agglutination behaviour of four-day-old epimastigote culture forms of 34 Trypanosoma cruzi, and T. cruzi-like stocks from Brazil, Chile, Colombia, Ecuador and Peru were tested with 15 carbohydrate-specific lectins. We distinguished intraspecifically two groups of agglutination reactions: Group 1 includes stocks which react with Triticum vulgaris and Aaptos papillata II (wheat germ agglutinin: WGA-type). Group 2 includes stocks agglutinated by Arachis hypogaea and Aaptos papillata II (peanut lectin: PNA-type). The agglutination reactions with lectins from Triticum vulgaris and Aaptos papillata II correlate with the presence of N-acetylneuraminic acid on the cell surface. After treatment with neuraminidase, the WGA-type is agglutinated by PNA but not by lectins from Triticum vulgaris and Aaptos papillata II. Further results demonstrate that a certain zymodeme pattern can be correlated with carbohydrate determinants.