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Related Experiment Videos

Experimental chimaerism in sheep.

C B Fehilly, S M Willadsen, E M Tucker

    Journal of Reproduction and Fertility
    |January 1, 1984
    PubMed
    Summary
    This summary is machine-generated.

    Creating composite sheep embryos from multiple early-stage embryos resulted in a high rate of chimeric lambs, especially when the composite embryo had a normal or greater cell number. This research advances understanding of early embryonic development and chimerism in mammals.

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    Area of Science:

    • Reproductive biology
    • Developmental biology
    • Animal science

    Background:

    • Chimerism, an organism with genetically distinct cells, is crucial for understanding developmental potential.
    • Sheep embryos offer a valuable model for studying mammalian chimerism due to their reproductive characteristics.

    Purpose of the Study:

    • To investigate the feasibility of creating composite sheep embryos by aggregating blastomeres from early-stage embryos.
    • To determine the rate of chimerism in lambs resulting from these composite embryos.
    • To assess the influence of cell number on chimerism rates.

    Main Methods:

    • Aggregation of blastomeres from 2-, 4-, or 8-cell sheep embryos to form composite embryos.
    • In vitro culture of composite embryos to the early blastocyst stage.

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  • Transfer of blastocysts to recipient ewes and assessment of lamb development and chimerism via blood typing and external features.

    • Main Results:

    • Successful development of normally organized blastocysts from 77% of composite embryos.
    • Thirty-eight recipients produced 53 lambs, with 36 (75%) identified as chimeras.
    • A higher proportion of chimeras (25/26) was observed in lambs from composite embryos with a normal or greater cell number compared to those with fewer cells (11/22).

    Conclusions:

    • Composite sheep embryo aggregation is an effective method for producing chimeric lambs.
    • The cell number of the composite embryo significantly influences the rate of chimerism.
    • This technique provides a robust model for studying developmental biology and genetic contribution in mammals.