One-step Plasmodium falciparum cultivation--application to in-vitro drug testing

P. falciparum was cultivated by a one-step method which requires medium renewal and addition of fresh RBC only every three days and allows high proliferation rates of the parasite. The method has been used for routine maintenance of strains and applied to the in-vitro evaluation of the effect of antimalarial compounds over 3 days periods as measured by 3H-Hypoxanthine incorporation.