Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Structural alterations of pathologically or physiologically modified DNA.

M Ciomei, S Spadari, G Pedrali-Noy

    Nucleic Acids Research
    |February 24, 1984
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    The major DNA polymerase in cultured plant cells: Partial purification and correlation with cell multiplication.

    Planta·2013
    Same author

    Inactivation of aphidicolin by plant cells.

    Plant cell reports·2013
    Same author

    Anti-tumour efficacy on glioma models of PHA-848125, a multi-kinase inhibitor able to cross the blood-brain barrier.

    British journal of pharmacology·2013
    Same author

    Molecular basis for the antiviral and anticancer activities of unnatural L-beta-nucleosides.

    Expert opinion on investigational drugs·2005
    Same author

    Serological study on Chlamydophila pneumoniae in patients with community-acquired pneumonia.

    The new microbiologica·2005
    Same author

    HIV-1 reverse transcriptase inhibitors: current issues and future perspectives.

    Current drug metabolism·2004
    Same journal

    Neurochondrin promotes U5 snRNP maturation by regulating AAR2 release from PRPF8.

    Nucleic acids research·2026
    Same journal

    Elongationless start-stop elements are stress-resilient translation gates that are more repressive than uTranslons.

    Nucleic acids research·2026
    Same journal

    Evolution of the ribosomal exit tunnel through the eyes of the nascent chain.

    Nucleic acids research·2026
    Same journal

    Enhancing the performance and interpretability of epigenetic clocks.

    Nucleic acids research·2026
    Same journal

    FABIAN-variant 2026: improved prediction of the effects of DNA variants on transcription factor binding.

    Nucleic acids research·2026
    Same journal

    Structural and biochemical characterization of Grimontia hollisae thermostable direct hemolysin with DNA reveals first Vibrio hemolysin with nuclease activity.

    Nucleic acids research·2026
    See all related articles

    Pathological DNA damage, like depurination, unwinds DNA, altering supercoiling. Physiological methylation does not affect DNA structure, suggesting distinct repair pathway recognition for DNA damage.

    Area of Science:

    • Molecular Biology
    • Biochemistry
    • Genetics

    Background:

    • DNA conformation and supercoiling are crucial for DNA replication and repair.
    • Pathological DNA modifications can alter DNA structure, potentially impacting cellular processes.
    • Understanding how DNA damage affects DNA topology is key to deciphering DNA repair mechanisms.

    Purpose of the Study:

    • To investigate the impact of depurination and methylation on DNA conformation.
    • To quantify the unwinding angle associated with specific DNA modifications.
    • To explore the implications for DNA damage recognition by repair enzymes.

    Main Methods:

    • In vitro analysis of topological isomers of plasmid pAT 153.
    • Induction of depurination (heat/acid) and methylation (methyl methanesulfonate, enzymatic).

    Related Experiment Videos

  • Assessment of DNA unwinding via changes in supercoiling and electrophoretic mobility on agarose gel.

    • Main Results:

    • Depurination and methyl methanesulfonate alkylation caused significant DNA unwinding, reducing supercoiling.
    • Enzymatic methylation at cytosine C-5 did not alter DNA tertiary structure.
    • Calculated unwinding angles: < -3.4° per methylated purine and ~ -12.0° per apurinic site.

    Conclusions:

    • Pathological DNA modifications induce structural changes (unwinding) detectable by supercoiling alterations.
    • Physiological methylation does not impact DNA tertiary structure.
    • DNA unwinding associated with various damage types may facilitate recognition by non-specific repair mechanisms.