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A simple method for immunoselective cell separation with the avidin-biotin system
A new method for immunoselective cell separation is described which uses biotinylated antibodies and avidin-coupled sheep erythrocytes for rosette formation, and a density gradient to separate the rosetting from the non-rosetting cells. The utility of the technique is illustrated by separating subpopulations of rabbit, mouse and rat lymphocytes. The method offers a rapid, sensitive and easily reproducible means for obtaining purified cell populations.
A new method for immunoselective cell separation is described which uses biotinylated antibodies and avidin-coupled sheep erythrocytes for rosette formation, and a density gradient to separate the rosetting from the non-rosetting cells. The utility of the technique is illustrated by separating subpopulations of rabbit, mouse and rat lymphocytes. The method offers a rapid, sensitive and easily reproducible means for obtaining purified cell populations.