Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Replication control of IncP plasmids.

N J Grinter

    Plasmid
    |January 1, 1984
    PubMed
    Summary
    This summary is machine-generated.

    Researchers studied Escherichia coli strains with integrated RP4-prime plasmids, observing a 50-fold increase in chromosome transfer. Plasmid copy number regulation suggests control by diffusible replication factors.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Two mechanisms necessary for the stable inheritance of plasmid RP4.

    Plasmid·1989
    Same author

    Replication defective RP4 plasmids recovered after chromosomal integration.

    Plasmid·1984
    Same author

    A broad-host-range cloning vector transposable to various replicons.

    Gene·1983
    Same author

    Analysis of chromosome mobilization using hybrids between plasmid RP4 and a fragment of bacteriophage lambda carrying IS1.

    Plasmid·1981
    Same author

    Conjugal transfer system of plasmid RP4: analysis by transposon 7 insertion.

    Journal of bacteriology·1978
    Same author

    Map of plasmid RP4 derived by insertion of transposon C.

    Journal of molecular biology·1977

    Area of Science:

    • Microbiology
    • Molecular Biology
    • Genetics

    Background:

    • Plasmids play crucial roles in bacterial genetics, mediating horizontal gene transfer and providing adaptive traits.
    • Understanding plasmid replication and maintenance is key to controlling genetic elements in bacteria.
    • Escherichia coli serves as a model organism for studying bacterial conjugation and plasmid biology.

    Purpose of the Study:

    • To investigate the effects of chromosomally integrated RP4-prime plasmids on Escherichia coli.
    • To analyze the impact of plasmid integration on chromosome transfer efficiency.
    • To elucidate the mechanisms regulating plasmid copy number in the presence of integrated elements.

    Main Methods:

    • Isolation and characterization of Escherichia coli clones harboring chromosomally integrated RP4-prime plasmids.

    Related Experiment Videos

  • Assessment of chromosome transfer frequencies using Hfr strains.
  • Elimination of autonomous plasmids using incompatible plasmids (pRP64, R751).
  • Quantification of plasmid copy numbers (integrated and autonomous).

    • Main Results:

    • Chromosome transfer increased approximately 50-fold in strains with integrated RP4-prime plasmids.
    • Integrated plasmids did not prevent the maintenance of autonomous plasmids.
    • Elimination of autonomous plasmids resulted in stable retention of resistance patterns and continued high chromosome transfer.
    • The total number of P plasmid genomes (integrated and autonomous) remained constant, with reduced autonomous plasmid copy number when integrated RP4 was present.

    Conclusions:

    • Chromosomally integrated plasmids can significantly enhance chromosome transfer in Escherichia coli.
    • Plasmid copy number appears to be regulated by a dosage-dependent mechanism, possibly involving diffusible replication inhibitors or initiators.
    • The findings support models of plasmid replication control influenced by the overall plasmid genome content within the cell.