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Saccharomyces cerevisiae CDC8 gene and its product
Molecular and Cellular Biology
|April 1, 1984
Summary
Researchers identified the Saccharomyces cerevisiae CDC8 gene product, crucial for DNA replication. This finding clarifies the gene
Area of Science:
- Molecular Biology
- Genetics
- Yeast Biology
Background:
- The Saccharomyces cerevisiae CDC8 gene is vital for cellular DNA replication.
- Understanding its gene product is key to elucidating its role in DNA replication.
Purpose of the Study:
- To clone and characterize the Saccharomyces cerevisiae CDC8 gene.
- To determine the structure and sequence of the CDC8 gene product.
Main Methods:
- Cloning a functional CDC8 gene fragment from S. cerevisiae.
- Nucleotide sequencing and analysis of the gene.
- RNA hybridization and in vitro translation to identify the gene product.
Main Results:
- A 1,000-base-pair fragment containing the functional CDC8 gene was cloned.
- The gene sequence predicts a 216-amino acid polypeptide with a molecular weight of 24,600.
- In vitro translation confirmed the predicted polypeptide size, identifying the authentic CDC8 gene product.
Conclusions:
- The amino acid sequence of the Saccharomyces cerevisiae CDC8 gene product has been deduced.
- This study provides a molecular characterization of the CDC8 gene product.
- Results differ from previous studies relying on in vitro complementation assays.