Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Human apolipoprotein B: partial amino acid sequence.

R C LeBoeuf, C Miller, J E Shively

    FEBS Letters
    |May 7, 1984
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Trimethylamine N-oxide levels are associated with NASH in obese subjects with type 2 diabetes.

    Diabetes & metabolism·2020
    Same author

    Heparin-binding EGF-like growth factor (HB-EGF) antisense oligonucleotide protected against hyperlipidemia-associated atherosclerosis.

    Nutrition, metabolism, and cardiovascular diseases : NMCD·2019
    Same author

    Flavin monooxygenase 3, the host hepatic enzyme in the metaorganismal trimethylamine N-oxide-generating pathway, modulates platelet responsiveness and thrombosis risk.

    Journal of thrombosis and haemostasis : JTH·2018
    Same author

    Heritability of periodontal bone loss in mice.

    Journal of periodontal research·2015
    Same author

    Zbtb16 has a role in brown adipocyte bioenergetics.

    Nutrition & diabetes·2013
    Same author

    The mouse model for atherosclerosis.

    Trends in cardiovascular medicine·2011

    Researchers developed a new method for sequencing apolipoprotein B using limited proteolysis and gel electrophoresis. This approach yielded large peptides, enabling partial sequencing and identification of potential oligonucleotide probe sites.

    Area of Science:

    • Biochemistry
    • Molecular Biology
    • Proteomics

    Background:

    • Apolipoprotein B (ApoB) is a crucial protein in lipoprotein metabolism.
    • Accurate sequencing of ApoB is essential for understanding its function and associated diseases.
    • Previous sequencing methods had limitations in efficiency and resolution.

    Purpose of the Study:

    • To develop an improved method for sequencing apolipoprotein B.
    • To identify specific peptide fragments of ApoB for further analysis.
    • To determine potential regions for oligonucleotide probe development.

    Main Methods:

    • Limited proteolysis of apolipoprotein B in the presence of sodium dodecyl sulfate.
    • Isolation of large peptides using preparative gel electrophoresis.

    Related Experiment Videos

  • Partial sequencing of isolated peptides via spinning-cup microsequencing.

    • Main Results:

    • Successfully generated large, relatively pure peptides from apolipoprotein B.
    • Partially sequenced two distinct peptides (R2-5 and R3-1) with identified amino acid residues.
    • Identified several residue stretches suitable for oligonucleotide probe construction.

    Conclusions:

    • The developed method provides a successful approach for apolipoprotein B sequencing.
    • The partial sequences offer valuable data for further structural and functional studies.
    • The identified probe sites facilitate the development of molecular tools for ApoB research.