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Updated: Jul 25, 2026

A Simple Behavioral Assay for Testing Visual Function in Xenopus laevis
Published on: June 12, 2014
Phototactic responses of anuran amphibians to monochromatic stimuli of equal quantum intensity
Abstract:
The phototactic responses of anuran amphibians to narrow-band monochromatic stimuli of equal quantum intensity were measured for the first time in eight new experiments. The unimodal spectral response, obtained from dark-adapted American toads (Bufo americanus), peaks near 626 THz of frequency (480 nm wavelength). The bimodal, U-shaped spectral response, obtained from dark-adapted tailed frogs (Ascaphus truei), has the anti-mode at about 589 THz (510 nm) and is not merely the spectral mirror-image of the unimodal response. Absolute level of the spectral stimuli of equal quantum intensity did not affect the spectral response of dark-adapted toads, but light-adaptation enhanced a component that has the same spectral peak as the visual pigment absorption spectrum of principal and single cones of the frog's retina.
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