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Tissue and gene specificity of mouse renin expression
Hypertension (Dallas, Tex. : 1979)
|July 1, 1984
Summary
Mouse strains with a duplicated renin gene (Ren-2) show significantly higher submaxillary gland renin levels. Kidney renin remains unaffected, but a genetic variation may influence renin glycosylation.
Area of Science:
- Genetics
- Biochemistry
- Molecular Biology
Background:
- The Ren-1 locus in mice encodes renin, crucial for angiotensin II production.
- Some mouse strains possess a duplicated renin gene (Ren-2) near Ren-1.
- Gene duplication impacts renin levels, particularly in the submaxillary gland.
Purpose of the Study:
- Investigate the effects of renin gene duplication on renin expression and glycosylation.
- Analyze renin mRNA transcripts in different tissues.
- Identify genetic variations contributing to differential renin processing.
Main Methods:
- Sequence analysis of renin cDNA from DBA/2Ha mice.
- Comparative analysis of renin levels in kidney and submaxillary glands.
- Tissue surveys for renin mRNA expression.
Main Results:
- Submaxillary gland renin levels are up to 100-fold higher in strains with Ren-2 duplication.
- Kidney renin levels are not significantly affected by gene duplication.
- A single nucleotide substitution in kidney renin mRNA creates a potential glycosylation site.
- Renin mRNA is detected in adrenal glands, testes, and salivary glands, with length variations.
Conclusions:
- Renin gene duplication primarily affects submaxillary gland renin levels.
- A specific base substitution may explain differential glycosylation of kidney and submaxillary renins.
- Renin gene expression is tissue-specific, with variations in transcript length observed.