Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Fixing Double-strand Breaks02:04

Fixing Double-strand Breaks

The double-stranded structure of DNA has two major advantages. First, it serves as a safe repository of genetic information where one strand serves as the back-up in case the other strand is damaged. Second, the double-helical structure can be wrapped around proteins called histones to form nucleosomes, which can then be tightly wound to form chromosomes. This way, DNA chains up to 2 inches long can be contained within microscopic structures in a cell. A double-stranded break not only damages...
Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart, a...
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
Fixing Double-strand Breaks02:04

Fixing Double-strand Breaks

The double-stranded structure of DNA has two major advantages. First, it serves as a safe repository of genetic information where one strand serves as the back-up in case the other strand is damaged. Second, the double-helical structure can be wrapped around proteins called histones to form nucleosomes, which can then be tightly wound to form chromosomes. This way, DNA chains up to 2 inches long can be contained within microscopic structures in a cell. A double-stranded break not only damages...
Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Flip-flop-induced relaxation of bending energy: implications for membrane remodeling.

Biophysical journal·2009
Same author

Reconstructing the emergence of cellular life through the synthesis of model protocells.

Cold Spring Harbor symposia on quantitative biology·2009
Same author

Is there variation in crossover interference levels among chromosomes from human males?

Genetics·2009
Same author

Semipermeable lipid bilayers exhibit diastereoselectivity favoring ribose.

Proceedings of the National Academy of Sciences of the United States of America·2005
Same author

Alfred Day Hershey.

Biographical memoirs. National Academy of Sciences (U.S.)·2004
Same author

Crossover interference in humans.

American journal of human genetics·2003

Related Experiment Video

Updated: Jul 10, 2026

Characterizing DNA Repair Processes at Transient and Long-lasting Double-strand DNA Breaks by Immunofluorescence Microscopy
08:31

Characterizing DNA Repair Processes at Transient and Long-lasting Double-strand DNA Breaks by Immunofluorescence Microscopy

Published on: June 8, 2018

The double-strand-break repair model for recombination.

J W Szostak, T L Orr-Weaver, R J Rothstein

    Cell
    |May 1, 1983
    PubMed
    Summary

    Gene conversion during meiosis, often linked to crossing-over, is explained by a new double-strand break repair model. This mechanism clarifies gene conversion and postmeiotic segregation, offering insights into genetic recombination.

    Area of Science:

    • Genetics
    • Molecular Biology
    • Cell Biology

    Background:

    • Gene conversion is the transfer of genetic information between DNA sequences.
    • Meiotic recombination, including crossing-over, is a fundamental genetic process.
    • Previous models explain gene conversion via single-strand nicks and heteroduplex DNA repair.

    Purpose of the Study:

    • To propose a novel mechanism for meiotic recombination.
    • To explain gene conversion and postmeiotic segregation through a new model.
    • To explore the genetic implications of the proposed recombination mechanism.

    Main Methods:

    • Review of existing genetic properties of meiotic recombination.
    • Analysis of previous models of gene conversion and crossing-over.

    More Related Videos

    Real-time Observation of the DNA Strand Exchange Reaction Mediated by Rad51
    06:24

    Real-time Observation of the DNA Strand Exchange Reaction Mediated by Rad51

    Published on: February 13, 2019

    Visualization of DNA Repair Proteins Interaction by Immunofluorescence
    07:55

    Visualization of DNA Repair Proteins Interaction by Immunofluorescence

    Published on: June 26, 2020

    Related Experiment Videos

    Last Updated: Jul 10, 2026

    Characterizing DNA Repair Processes at Transient and Long-lasting Double-strand DNA Breaks by Immunofluorescence Microscopy
    08:31

    Characterizing DNA Repair Processes at Transient and Long-lasting Double-strand DNA Breaks by Immunofluorescence Microscopy

    Published on: June 8, 2018

    Real-time Observation of the DNA Strand Exchange Reaction Mediated by Rad51
    06:24

    Real-time Observation of the DNA Strand Exchange Reaction Mediated by Rad51

    Published on: February 13, 2019

    Visualization of DNA Repair Proteins Interaction by Immunofluorescence
    07:55

    Visualization of DNA Repair Proteins Interaction by Immunofluorescence

    Published on: June 26, 2020

  • Proposal and exploration of a double-strand break repair model for recombination.

    • Main Results:

    • A new model initiating recombination with double-strand breaks (enlarged to gaps) is proposed.
    • Gene conversion is explained by the repair of double-strand gaps.
    • Postmeiotic segregation arises from heteroduplex DNA at gap-repair boundaries.

    Conclusions:

    • The double-strand break repair model provides a unified explanation for gene conversion and crossing-over.
    • This model is supported by efficient double-strand gap repair in yeast.
    • The proposed mechanism offers new perspectives on the genetic regulation of meiosis.