Interaction of purified rat liver glucocorticoid-receptor complexes with polynucleotides: strong base composition...

The binding of 10(3)-fold purified rat liver glucocorticoid receptors to natural and synthetic polynucleotides has been studied. The receptors may bind to RNA as well as to DNA. The binding effectiveness of nucleic acids is strongly base composition dependent, with natural DNAs (double-stranded or denatured) greater than E. coli rRNA greater than poly(dA).poly(dT) greater than or equal to poly d(A,T,G)7 greater than or equal to poly(U) greater than poly(A).poly(U) greater than poly(A) approximately poly(C). The equilibrium (apparent) constants of receptor binding to a number of polynucleotides were calculated by a method proposed.