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Isolation and nucleotide sequence of a cDNA encoding human calmodulin.

E J Wawrzynczak, R N Perham

    Biochemistry International
    |August 1, 1984
    PubMed
    Summary

    Researchers isolated a human calmodulin cDNA clone, revealing its complete coding sequence and mRNA flanking regions. This finding provides a crucial tool for studying calmodulin

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    Area of Science:

    • Molecular Biology
    • Genetics

    Background:

    • Calmodulin is a vital calcium-binding protein involved in numerous cellular processes.
    • Understanding calmodulin's genetic basis is essential for deciphering its regulatory roles.

    Purpose of the Study:

    • To isolate and determine the nucleotide sequence of a human calmodulin cDNA clone.
    • To obtain the complete coding sequence and flanking regions of the calmodulin mRNA.

    Main Methods:

    • Screening of a cDNA library using mixed oligodeoxyribonucleotide probes.
    • Nucleotide sequencing of the isolated cDNA clone.

    Main Results:

    • Isolation of a cDNA clone containing the entire coding sequence of human calmodulin.
    • Identification of substantial portions of the untranslated flanking regions of the mRNA.
    • Predicted primary structure matches known calmodulin, with an additional N-terminal methionine.

    Conclusions:

    • The isolated cDNA clone represents the full coding sequence of human calmodulin.
    • This clone serves as a valuable resource for further molecular and genetic studies of calmodulin.
    • The N-terminal methionine suggests the initiation site of calmodulin translation.

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