Improved fixation for immunofluorescence microscopy using light-activated 1,3,5-triazido-2,4,6-trinitrobenzene (TTB)

A novel fixation method using 1,3,5-triazido-2,4,6-trinitrobenzene (TTB) improves cell morphology and antigen preservation for immunofluorescence microscopy. This TTB method enhances image quality, particularly for cytoskeletal proteins, offering a superior alternative to conventional techniques.