A simple method of analyzing profiles in time-difference direct optical scanning gel chromatography

A simple method is presented for the analysis of time-difference, large-zone chromatography profiles. The method is derived from basic theory and is applicable to multicomponent as well as single-component systems. Simple computer simulations are used to demonstrate the inaccuracies of earlier, more empirical methods. This method has been tested on several proteins using an inexpensive, semi-automated, data acquisition and control system.