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"PMN-elastase assay": enzyme immunoassay for human polymorphonuclear elastase complexed with alpha 1-proteinase
Journal of Clinical Chemistry and Clinical Biochemistry. Zeitschrift Fur Klinische Chemie Und Klinische Biochemie
|October 1, 1984
Summary
A new enzyme-linked immunoassay accurately measures human granulocyte elastase (HGE) complexed with alpha 1-proteinase inhibitor in plasma. This assay is valuable for diagnosing inflammatory diseases.
Area of Science:
- Biochemistry
- Immunology
- Clinical Chemistry
Background:
- Human granulocyte elastase (HGE) is a key protease involved in inflammatory processes.
- The complex of HGE with alpha 1-proteinase inhibitor (alpha 1-PI) is a relevant biomarker in inflammation.
- Accurate quantification of this complex is crucial for understanding disease pathogenesis.
Purpose of the Study:
- To develop and validate a solid-phase, enzyme-linked immunoassay (ELISA) for the quantitative determination of the HGE-alpha 1-PI complex.
- To assess the suitability of the assay for routine use in clinical chemistry laboratories.
- To establish a reference range for HGE levels in plasma and discuss its diagnostic significance.
Main Methods:
- Solid-phase enzyme-linked immunoassay using antibody-coated test tubes.
- Quantitative determination of the complex of human granulocyte elastase (EC 3.4.21.37) with alpha 1-proteinase inhibitor.
- Evaluation of sample stability and test characteristics.
Main Results:
- A reliable ELISA assay for quantifying the HGE-alpha 1-PI complex was developed.
- The assay demonstrated suitability for routine clinical chemistry laboratory use.
- A reference range of 20-180 micrograms/l for elastase in plasma was established.
Conclusions:
- The developed ELISA provides a robust method for measuring HGE-alpha 1-PI complex in plasma.
- This assay has significant diagnostic potential for inflammatory diseases.
- Further studies can utilize this assay to explore the role of HGE in various pathological conditions.