[Study on renal transplantation. IX. Experimental study on kidney preservation with perfluorochemical emulsion at low...

The unilateral kidney of mongrel dog was removed and flushed out with modified Collins C3 solution, and then preserved by pulsatile perfusion machine (MOX-100) at low temperature of 4-6 degrees C. During the perfusion, systolic pressure was controlled below 60 mmHg. After a 48-hr or 72-hr preservation, the kidney was autotransplanted to the iliac fossa of the same dog. The perfusate consisted of cryoprecipitated plasma (CPP) of the dogs supplemented with or without 5 W/V% perfluorochemical emulsion (FC-43). At an early stage, our CPP formed fibrin mass easily, thus being very unstable. But silicagel treatment of the CPP improved stability. Twenty six kidneys were perfused for preservation, but perfusion of 7 kidneys was stopped by decreased perfusion flow and increased perfusion pressure. Eight kidneys were lost due to technical failure of transplantation or infection after autotransplantation; 11 kidneys were used as planned. The 48-hr preservation group contained 2 cases of FC (+) and 2 cases of FC (-). Each group had one survival case. The 72-hr preservation group contained 6 cases of FC (+), and one case of FC (-). Three cases survived in the FC (+) group and one case survived in the FC (-) group. Survival cases maintained enough perfusion flow which was above 0.3 ml/min. g. kidney. Weight gains of the preserved kidneys during 72-hr preservation were 13 +/- 19% in FC (+) and 21 +/- 17% in FC (-) group, but had no relation to preservation condition.(ABSTRACT TRUNCATED AT 250 WORDS)