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[Methods for testing surface disinfection procedures under current conventional conditions].

R Andenmatten, H Reber

    Zentralblatt Fur Bakteriologie, Mikrobiologie Und Hygiene. 1. Abt. Originale B, Hygiene
    |August 1, 1984
    PubMed
    Summary
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    This study introduces a practical method for testing surface disinfectants. The new method quantifies germ reduction on surfaces and in cleaning water, proving effective for evaluating disinfectant performance.

    Area of Science:

    • Microbiology
    • Environmental Science
    • Public Health

    Background:

    • Surface disinfection is crucial for preventing pathogen transmission in healthcare and public settings.
    • Standardized testing methods are needed to accurately assess disinfectant efficacy under realistic conditions.

    Purpose of the Study:

    • To develop and validate a quantitative method for testing surface disinfection procedures that mimics real-world application.
    • To evaluate the efficacy of disinfectants by measuring germ reduction on surfaces and in used cleaning water.

    Main Methods:

    • A standardized wet-mopping procedure using a two-bucket system on glazed clinker floors was employed as a reference.
    • The test involved applying disinfectants conventionally and measuring the reduction in colony count (CC) of specific bacterial strains (Kl. pneumoniae, Staph. aureus) on surfaces and in washing water.

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  • Bacterial extraction from surfaces was performed using a bacterial collector after Thran.

    • Main Results:

    • Wet mopping with standardized hard water reduced Kl. pneumoniae by 2.8 log and Staph. aureus by 2.2 log.
    • Disinfectants further reduced bacterial counts by 0.45–0.72 log (Kl. pneumoniae) and 1.2–2.2 log (Staph. aureus).
    • Analysis of washing water revealed significant germ reduction (1.8–3.7 log for Kl. pneumoniae, 3.1–3.7 log for Staph. aureus) after 5 minutes, with continued reduction over 30 minutes.

    Conclusions:

    • The proposed testing method accurately reflects practical surface disinfection scenarios.
    • Analyzing germ reduction in washing water is a key parameter for evaluating disinfectant effectiveness.
    • The flexible testing procedure can be adapted for various disinfection challenges and evaluation criteria.