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Published on: December 12, 2011

Enzyme-immunoassay: a review

M Oellerich

    Journal of Clinical Chemistry and Clinical Biochemistry. Zeitschrift Fur Klinische Chemie Und Klinische Biochemie
    |December 1, 1984
    PubMed

    The main principles of heterogeneous and homogeneous enzyme-immunoassays are reviewed. Furthermore, an overview is given of enzymes currently used as labels and methods applied for conjugate preparation. The reliability and practicability of enzyme-immunoassays are discussed. Special consideration is given to possible interferences, the detection limits and the mechanization of these assays. A range of curve-fitting methods for evaluation of the results is listed. Future prospects for the further development of enzyme-immunoassay and application of this technique are discussed.

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