Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Enzyme-immunoassay: a review.

M Oellerich

    Journal of Clinical Chemistry and Clinical Biochemistry. Zeitschrift Fur Klinische Chemie Und Klinische Biochemie
    |December 1, 1984
    PubMed
    Summary
    This summary is machine-generated.

    This review covers enzyme-immunoassays (EIAs), detailing enzyme labels, conjugate preparation, and assay reliability. It also discusses interferences, detection limits, mechanization, and future prospects for EIA development.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    What do we need to obtain high quality circulating tumor DNA (ctDNA) for routine diagnostic test in oncology? - Considerations on pre-analytical aspects by the IFCC workgroup cfDNA.

    Clinica chimica acta; international journal of clinical chemistry·2021
    Same author

    Donor-Derived Cell-Free DNA Is a Novel Universal Biomarker for Allograft Rejection in Solid Organ Transplantation.

    Transplantation proceedings·2015
    Same author

    Differential S-nitrosylation of proteins in Alzheimer's disease.

    Neuroscience·2013
    Same author

    CD4+ immune response as a potential biomarker of patient reported inflammatory bowel disease (IBD) activity.

    Clinica chimica acta; international journal of clinical chemistry·2013
    Same author

    Inosine monophosphate dehydrogenase activity in paediatrics: age-related regulation and response to mycophenolic acid.

    European journal of clinical pharmacology·2012
    Same author

    Population pharmacokinetics of mycophenolic acid in pediatric renal transplant patients using parametric and nonparametric approaches.

    Pharmacological research·2010
    Same journal

    Determination of amniotic fluid acetylcholinesterase activity in the antenatal diagnosis of foetal malformations: the first ten years.

    Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie·1990
    Same journal

    Antibodies as a source of analytical errors.

    Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie·1990
    Same journal

    Determination of methotrexate in serum: the aca MTHO immunoassay and HPLC compared.

    Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie·1990
    Same journal

    Improved sample and reagent pipetting with the MINILAB.

    Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie·1990
    Same journal

    Enzyme immunoassay of testosterone using nitrocellulose discs as the solid phase.

    Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie·1990
    Same journal

    Interference of immunoglobulins in the radioimmunoassay of human beta-endorphin.

    Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie·1990
    See all related articles

    Area of Science:

    • Biochemistry
    • Immunology
    • Analytical Chemistry

    Background:

    • Enzyme-immunoassays (EIAs) are widely used analytical techniques.
    • Understanding EIA principles is crucial for accurate diagnostics.

    Purpose of the Study:

    • To review the fundamental principles of heterogeneous and homogeneous EIAs.
    • To provide an overview of enzymes used as labels and conjugate preparation methods.
    • To discuss the reliability, practicability, and future of EIAs.

    Main Methods:

    • Review of existing literature on enzyme-immunoassays.
    • Discussion of enzyme labels and conjugate preparation techniques.
    • Analysis of assay performance parameters like detection limits and interferences.

    Related Experiment Videos

    Main Results:

    • Detailed review of homogeneous and heterogeneous EIA principles.
    • Overview of commonly used enzyme labels and conjugation strategies.
    • Discussion on factors affecting EIA reliability and practicability, including interferences and detection limits.

    Conclusions:

    • Enzyme-immunoassays are reliable and practical diagnostic tools.
    • Further development and application of EIAs are anticipated.
    • Optimization of curve-fitting methods enhances result evaluation.