Further developed device for human sperm freezing by the twenty-minute method

Area of Science:

• Reproductive Medicine
• Cryobiology
• Biomedical Engineering

Background:

• Sperm cryopreservation is crucial for assisted reproductive technologies.
• Existing methods can be time-consuming and complex.
• Need for efficient and practical sperm freezing devices.

Purpose of the Study:

• To introduce a novel, practical device for human sperm freezing.
• To simplify the sperm preparation and cryopreservation process.
• To evaluate the efficacy and outcomes of the new freezing device.

Main Methods:

• Device utilizes liquid nitrogen evaporation for controlled freezing.
• Thermostat control ensures precise temperature regulation.
• Accommodates both rapid-rate (pellets) and slow-rate (paillettes) freezing.
• Interchangeable freezing plates allow for sample isolation.

Main Results:

• Procedure time reduced to approximately 20 minutes.
• Freezing stages at +5°C and -80°C, with storage at -196°C.
• Achieved a sperm recovery index of 50% to 70%.
• Applied to 56 patients, resulting in 19 normal deliveries, 3 abortions, and 9 ongoing pregnancies.

Conclusions:

• The developed device offers a simplified and efficient method for human sperm cryopreservation.
• The device demonstrates clinical applicability with successful pregnancy and delivery outcomes.
• This practical approach can enhance the accessibility and success rates of fertility treatments.