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Ly-5 molecular forms in congenitally immunodeficient mice
M A Pierotti1, C Traversari, D Ballinari
1Division of Experimental Oncology A, National Tumor Institute, Milan, Italy.
Experimental and Clinical Immunogenetics
|January 1, 1984
Summary
This study identified four Ly-5 antigen forms on mouse spleen cells, each linked to specific cell types like B cells and macrophages. One form
Area of Science:
- Immunology
- Cell Biology
Background:
- The Ly-5 antigen is a cell surface protein found on various hematopoietic cells.
- Different molecular weight forms of Ly-5 may indicate distinct cellular functions or lineages.
Purpose of the Study:
- To characterize the different molecular weight forms of the Ly-5 antigen on murine spleen cells.
- To investigate the lineage-specific expression of these Ly-5 forms.
- To explore the potential role of Ly-5 in immune responses, particularly in immunodeficient models.
Main Methods:
- Immunochemical analysis using a monoclonal antibody to detect Ly-5 antigen.
- Analysis of cell surface expression on murine spleen cells, including B cells, macrophages, and thymocytes.
- Comparison of Ly-5 expression in normal mice versus immunodeficient mice (athymic nude, LASAT).
Main Results:
- Four distinct molecular weight forms of Ly-5 (220,000, 200,000, 185,000, and 175,000 daltons) were identified.
- The 220,000-dalton form was associated with B cells.
- The 200,000-dalton form was associated with macrophages.
- The 185,000 and 175,000-dalton forms were detected on normal thymocytes.
- The 185,000-dalton Ly-5 molecule was absent on lymphoid cells of immunodeficient mice.
Conclusions:
- Ly-5 antigen exhibits lineage-specific expression on murine spleen cells.
- The presence of the 185,000-dalton Ly-5 molecule on thymocytes suggests its involvement in T-dependent immune responses.
- Further investigation into the function of this specific Ly-5 form is warranted.