Related Experiment Videos
Multilayer film analysis: evaluation of ion-selective electrolyte slides
Clinical Chemistry
|January 1, 1983
Summary
The Kodak Ektachem multilayer slides offer precise electrolyte measurements for sodium, potassium, chloride, and carbon dioxide. Analytical recovery is generally accurate, with interchangeable use of serum or heparin plasma, though high protein affects sodium results.
Area of Science:
- Clinical Chemistry
- Medical Diagnostics
- Analytical Chemistry
Background:
- Ion-selective electrode (ISE) technology is crucial for electrolyte analysis in clinical settings.
- The Kodak Ektachem system offers a multilayer slide approach for various biochemical assays.
- Accurate electrolyte measurements are vital for patient diagnosis and management.
Purpose of the Study:
- To evaluate the performance of Kodak Ektachem multilayer ion-selective electrolyte slides.
- To assess the precision, accuracy, and reliability of electrolyte measurements using this system.
- To compare Ektachem results with established continuous-flow methods.
Main Methods:
- Evaluation of Kodak Ektachem multilayer ion-selective electrolyte slides.
- Determination of within- and between-day precision (CV) for sodium, potassium, carbon dioxide, and chloride.
- Assessment of analytical recovery across different concentrations.
- Comparison of Ektachem results with continuous-flow procedures.
- Investigation of the impact of sample matrix (serum, heparin plasma) and interfering substances (protein, lipemia).
Main Results:
- Excellent precision (CV 0.5-1.6%) for sodium, potassium, and chloride; acceptable precision (CV 2.9-4.6%) for carbon dioxide.
- Analytical recovery near 100% for most analytes, with higher recovery for carbon dioxide in supra-physiological ranges.
- Good correlation with continuous-flow methods for sodium, potassium, and chloride.
- No significant interference from low protein or lipemia; slight effect on sodium from high protein.
- Heparinized plasma and serum are interchangeable, but lithium iodoacetate-treated serum is unsuitable.
Conclusions:
- Kodak Ektachem multilayer ion-selective electrolyte slides provide reliable and precise measurements for key electrolytes.
- The system demonstrates good analytical recovery and minimal interference from common sample variations.
- Potential need for calibrator adjustment for carbon dioxide analysis to align with continuous-flow methods.